Ultrafine Bubbles Water priming to improve viability and vigor of bean (Phaseolus vulgaris) seeds
DOI:
https://doi.org/10.29244/jtcs.10.1.8-16Keywords:
germination, pre-planting, pre-storage, seed storability, seed qualityAbstract
Priming is a physiological technique of hydration of seed to improve metabolic processes before germination to accelerate germination and seedling growth under normal and stress conditions. This research is aimed to study the pretreatment using ultra-fine bubble (UFB) water to increase viability and vigor of seeds before planting (pre-planting) and during storage (pre-storage). This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia. The research consisted of two experiments, i.e., UFB water priming to increase viability and vigor of bean seeds and its storability after priming. The first experiment was arranged using a completely randomized design with a combination of seed lot treatments (L1: initial germination percentage (GP) about 80%, L2: initial GP around 70%, and L3: initial GP around 60%) with priming (P0: no priming/control, P1: soaked in distilled water for 60 minutes, P2: distilled water for 120 minutes, P3: 8 ppm UFB water for 60 minutes, P4: 8 ppm UFB water for 120 minutes, P5: 20 ppm UFB water for 60 minutes, P6: UFB water 20 ppm for 120 minutes). The second experiment was arranged using a nested design with the main factor being the condition of the storage room and priming as the second factor nested in the main factor. The storage room condition factors consisted of KM: room conditions (temperature 28±5˚C, RH 73±7%) and AC: air-conditioned room (18±2 ̊C, RH 61±7%). The priming factor consisted of two levels, namely P0: without priming and P1: priming with 20 ppm UFB water soaked for 120 minutes. The results showed that priming treatment with UFB water 20 ppm for 120 minutes as a pre-planting treatment can increase the viability of bean seeds, particularly for seeds with low initial viability, and primed bean seeds with an initial viability of approximately 80% were able to retain their viability for 16 weeks of storage in an air-conditioned room.
References
Adhinugraha, Q.S., Widajati, E., and Palupi, E.R., (2022). Invigoration improved quality and storability of the true seed of shallot (Allium ascalonicum). Journal of Tropical Crop Science 9, 145-155.
Butler, L.H., Hay, F.R., Ellis, R.H., Smith, R.D., and Murray, T.B. (2009). Priming and re-drying improve the survival of mature seeds of digitalis purpurea during storage. Annals of Botany 103, 1261-1270. DOI: 10.1093/AOB/mcp059
Fata, N.A.N., Supriyanto, N., Rustam, E., and Sudrajat, D.J., (2020). Invigoration treatment of white jabon (Neolamarckia cadamba (Roxb.) Bosser) seeds using polyethylene glycol and ultrafine bubbles. Jurnal Perbenihan Tanam Hutan 8, 11–24. DOI:10.20886/bptpth.2020.8.1.11-24
Gomes, M.P., and Garcia, Q.S., (2013). Reactive oxygen species and seed germination. Biologia 68, 351-57. DOI:10.2478/s11756-013-0161-y
Hill, H.J., Bradford, K.J., Cunningham J.D., and Taylor, A.G., (2008). Primed lettuce seeds exhibit increased sensitivity to moisture during aging. Acta Horticulturae 782,135-141. DOI:10.17660/ActaHortic.2008.782.14
Hussein, S., Zheng, M., Khan, F., Khaliq, A., Farhad, S., Peng, S., Huang, J., Cui, K., and Nie, L. (2015). The benefit of rice seed priming is offset permanently by prolonged storage and storage conditions. Scientific Reports 5, 1-12. DOI: 10.1038/srep08101
Ilyas, S. (2012). “Ilmu dan Teknologi Benih: Teori dan Hasil-Hasil Penelitian”. IPB Press.
Ishibashi, Y., Tawaratsumida, T., Zheng, S.H., Yuasa, T., and Iwaya, I.M. (2009). NADPH oxidases act as a key enzyme in barley germination and seedling growth (Hordeum vulgare L.). Plant Production Science 13, 45–52. DOI: 0.1626/pps.13.45
Liu, S., Oshita, S., Makino, Y., Wang, Q., Kawagoe, Y., and Uchida, T. (2016). Oxidative capacity of nanobubbles and its effect on seed germination. ACS Sustainable Chemistry and Engineering 4, 1347–1353. DOI:10.1021/acssuschemeng.5b01368
Malek, M., Ghaderi-Far, F., Torabi, B., Sadeghipour, H.R., and Hay, F.R. (2019). The influence of seed priming on storability of rapeseed (Brassica napus) seeds. Seed Science and Technology 47, 87-92. DOI: 10.15258/sst.2019.47.1.09
Mohajeri, F., Ramroudi, M., Taghvaei, M., Galavi, M. (2016). Effect of priming duration and concentration on germination behaviors of (Phaseolus vulgaris L.) seeds. Ecology, Environment and Conservation 22, 603-609
Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G.A., Sajid, M., Subtain, M.U., and Shabbir, I. (2013). Seed priming a technique. International Journal of Agriculture and Crop Sciences 6, 1373-1381.
Powell, A.A., Yule, L.J., Hai-Chun, J., Groot, S.P.C., Bino, R.J., and Pritchard, H.W. (2000). The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations. Journal of Experimental Botany 51, 2031-2043
Purwanto, Y.A., Maulana, N.N., and Naibaho, N. (2019). Effect of ultrafine bubbles water on seed germination. IOP Conference Series: Earth Environmental Science 355, 1-5. DOI: 10.1088/1755-1315/355/1/012073
Siregar, I.Z., Muharam, K.F., Purwanto, Y.A., and Sudrajat, D.J. (2020). Seed germination characteristics in different storage times of Gmelina arborea treated with ultrafine bubbles priming. Journal of Biological Diversity 21, 4558-4564. DOI: 10.13057/bio div/d211013
Tomizawa, S., Imai, H., Tsukada, S., Simizu, T., Honda, F., Nakamura, M., Nagano, T., Urano, Y., Matsuoka, Y., Fukasaku, N., and Saito, N. (2005). The detection and quantification of highly reactive oxygen species using the novel HPF fluorescence probe in a rat model of focal cerebral ischemia. Neuroscience Research 53, 304-313. DOI: 10.1016/j.neures.2005.08.002
Utami, E.P., Sari, M., and Widajati, E. (2013). Perlakuan priming benih untuk mempertahankan vigor benih kacang panjang (Vigna unguiculata) selama penyimpanan. Buletin Agrohorti 1, 75–82. DOI: 10.29244/agrob.1.4.75-82
Wang, W., He, A., Peng, S., Huang, J., Cui, K., and Nie, L. (2018). The effect of storage condition and duration on the deterioration of primed rice seeds. Frontiers in Plant Science 9, 1-17. DOI: 10.3389/fpls.2018.00172
Waqas, M., Korres, N.E., Khan, M.D., Nizami, A.S., Deeba, F., Ali, I., and Hussain, H. (2019). "Advances in the concept and methods of seed priming." Springer Nature Singapore Pte Ltd. 11– 43. DOI: 10.1007/978-981-13-8625-1_2
Wood, L.P., and Hay, F.R. (2010). Priming increases the storability and changes the water sorption properties of Rhododendron griersonianum seeds. Seed Science and Technology 38, 682-691. DOI:10.15258/sst.2010.38.3.16
Yan, M. (2017). Prolonged storage reduced the positive effect of hydropriming in Chinese cabbage seeds stored at different temperatures. South African Journal of Botany 111, 313–315. DOI:10.1016/j.sajb.2017.04.005
Yuanasari, B.S., Kendarini, N., and Saptadi, D. (2015). Peningkatan viabilitas benih kedelai hitam (Glycine max L. Merr) melalui invigorasi osmoconditioning. Jurnal Produksi Tanaman 3, 518-527.
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