Purple Cleome (Cleome rutidosperma) Growth, Yield, and Total Flavonoid Under Different Media Compositions and Harvesting Frequencies
DOI:
https://doi.org/10.29244/jtcs.13.01.160-173Keywords:
functional vegetables, organic, phytochemicals, traditional medicineAbstract
Purple cleome (Cleome rutidosperma) is a medicinal plant known for its flavonoid content, which confers multiple pharmacological benefits. However, cultivation methods to optimize both biomass production and bioactive compound accumulation remain underdeveloped. This study aimed to assess the influence of planting media composition and harvesting frequency on the growth, yield, and total flavonoid concentration of purple cleome. The experiment was conducted in Gunung Batu, Bogor, Indonesia, from January to April 2025, employing a two-factor randomized block design. The first factor was planting media composition, with four treatments: 100% soil (0) and soil mixed with cow manure at ratios of 1:1, 1:2, and 1:3 (v/v). The second factor was harvesting frequency, with two levels: A single harvest at 8 weeks after planting (WAP) and two harvests at 4 and 8 WAP. Results indicated that all tested media compositions supported plant growth effectively. Notably, total flavonoid concentration was significantly higher at 4 WAP compared to 8 WAP. Moreover, cumulative flavonoid yield from two harvests was significantly greater, reaching 5,639.80 mg quercetin equivalents (QUE) per plant dry weight, compared to 1,456.50 mg QUE per plant from a single harvest. These findings suggest that harvesting at multiple intervals enhances flavonoid yield in purple cleome cultivation.
References
Aparna, V., Dileep, K. V., Mandal, P. K., Karthe, P., Sadasivan, C., & Haridas, M. (2012). Anti-inflammatory property of n-hexadecanoic acid: structural evidence and kinetic assessment. Chemical Biology and Drug Design, 8(3), 434–439. http://doi.org/10.1111/j.1747-0285.2012.01418.x
Abubacker, M. N., & Deepalakshmi, T. (2013). In vitro antifungal potentials of bioactive compound methyl ester of hexadecanoic acid isolated from Annona muricata Linn. (Annonaceae) Leaves. Biosciences Biotechnology Research Asia, 10(2), 879 884. http://dx.doi.org/10.13005/bbra/1211
Al-suhaibani, N., Selim, M., Alderfasi, A., & El-hendawy, S. (2020). Comparative performance of integrated management between nutrient composted agricultural wastes, chemical fertilizers, and biofertilizers in improving soil quantitative and qualitative properties and crop yields under arid conditions. Agronomy, 10(1503), 1–25. http://doi.org/10.3390/agronomy10101503
Akinsola, A., & Oluwafemi, A. (2022). Phytochemical analysis, proximate composition, and mineral profile of Cleome rutidosperma found in Ado-Ekiti, Nigeria. International Journal of Advanced Biochemistry Research, 6(2), 94–100. https://doi.org/10.33545/26174693.2022.v6.i2b.141
Andharesta, A. B., & Sundari. (2024). Effect of cow manure and plant growth-promoting rhizobacteria on the yield of common bean. Jurnal Ilmu Pertanian Indonesia, 29(3), 435–446. https://doi.org/10.18343/jipi.29.3.435
Ali, N., Jiang, Q., Akhtar, K., Luo, R., Jiang, M., He, B., & Wen, R. (2025). Co-application of biochar and manure improves soil health and rice productivity by modulating microbial activity. BMC Plant Biology, 25(914), 2-17. https://doi.org/10.1186/s12870-025-06834-x
Bhardwaj, M., Sali, V. K., Mani, S., & Vasanthi, H. R. (2020). Neophytadiene from turbinaria ornata suppresses LPS-induced inflammatory response in RAW 264.7 macrophages and Sprague Dawley rats. Inflammation, 43(3), 1-14. https://doi.org/10.1007/s10753-020-01179-z
Bakrim, S., Benkhaira, N., Bourais, I., Benali, T., Lee, L., Omari, N. El, Sheikh, R. A., Goh, K. W., Ming, L. C., & Bouyahya, A. (2022). Health benefits and pharmacological properties of stigmasterol. Antioxidants, 11(10), 1–32. https://doi.org/10.3390/antiox11101912
Bermúdez, M. A., Pereira, L., Fraile, C., Valerio, L., Balboa, M. A., & Balsinde, J. (2022). Roles of palmitoleic acid and its positional isomers, hypogeic and sapienic acids, in inflammation, metabolic diseases, and cancer. Cells, 11(14), 1-16. https://doi.org/10.3390/cells11142146
Bai, X., Lu, W., Xu, J., Li, Q., & Xue, Z. (2025). Effects of cattle manure and sludge vermicompost on nutrient dynamics and yield in strawberry cultivation with distinct continuous cropping histories in a greenhouse. Frontiers in Plant Science, 10(15), 1–18. https://doi.org/10.3389/fpls.2024.1514675
Chen, Y., Lu, W., Chen, X., Zhang, J., Yang, K., Duan, X., Li, M., Kuang, M., Liu, S., Wu, X., Zou, G., Liu, C., Hong, C., He, W., Liao, J., Hou, C., Zhang, Z., Zheng, Q., Chen, J., Nanshan, Z., Makino, A., & Wang, J. (2020). Tetramethylpyrazine : A promising drug for the treatment of pulmonary hypertension. The British Pharmacological Society, 177(12), 2743–2764. https://doi.org/10.1111/bph.15000
Demonty, E. A. T. I. (2007). Phytosterols : natural compounds with established and emerging health benefits. Oleagineux Corps gras Lipides, 14(5), 259–266. http://dx.doi.org/10.1051/ocl.2007.0145
Fan, H., Huang, W., Guo, Y., & Ma, X. (2022). α-Linolenic acid suppresses proliferation and invasion in osteosarcoma cells via inhibiting fatty acid synthase. Molecules, 27(9), 1–13. https://doi.org/10.3390/molecules27092741
Ghosh, P., Chatterjee, S., Das, P., & Banerjee, A. (2019). Natural Habitat, phytochemistry, and pharmacological properties of a medicinal weed-cleome. International Journal of Pharmaceutical Sciences and 1605–1612. Research, 10(4), https://doi.org/10.13040/IJPSR.0975-8232.10(4).1605-12
Han, Z., He, W., Shi, H., Wang, C., Liu, C., & Feng, Y. (2023). Effects of the integrated use of dairy cow manure on soil properties and biological fertility. Sustainability, 15(15), 1-16. https://doi.org/10.3390/su151511693
Houdegbe, A. C., Achigan-Dako, E. G., Sogbohossou, E. O. D., Schranz, M. E., Odindo, A. O., & Sibiya, J. (2022). Leaf elemental composition analysis in spider plant [Gynandropsis gynandra L. (Briq.)] differentiates three nutritional groups. Frontiers in Plant Science, 13(841226), 1–21. https://doi.org/10.3389/fpls.2022.841226
Ikhwan, A., Indratmi, D., Hasanah, F., Atoum, M. F. M., & Iqrar, I. (2021). Analysis of metabolites from purple Cleome extract (Cleome rutidosperma Linn.) as potential organic fungicides. Sarhad Journal of Agriculture, 37(1), 115–121. https://doi.org/10.17582/JOURNAL.SJA/2021/37.S1.115.121
Jamiołkowska, A. (2020). Natural compounds as elicitors of plant resistance against diseases and new biocontrol strategies. Agronomy, 10(2), 1-11. https://doi.org/10.3390/agronomy10020173
Karimuna, S. R., Aziz, S. A., & Melati, M. (2015). Correlations between leaf nutrient concentration and the production of metabolites in orange jessamine (Murraya paniculata L. Jack) fertilized with chicken manure. Journal of Tropical Crop Science, 2(1), 16–25. http://doi.org/10.29244/jtcs.2.1.16-25
Kalu, O., Chimezie, E., & Mensah, S. (2017). Micromorphological and phytochemical studies on Cleome rutidosperma Linn. Journal of Advances in Biology & Biotechnology, 11(3), 1–8. https://doi.org/10.9734/jabb/2017/31028
Kim, E., Trang, N. M., Kang, H., & Kim, K. H. (2022). Phytol suppresses osteoclast differentiation and oxidative stress through Nrf2/HO-1 regulation in RANKL-induced RAW264.7. Cells, 11(22), 1–16. https://doi.org/10.3390/cells11223596
Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: An overview. The Scientific World Journal, 2013(16), 1-16. http://dx.doi.org/10.1155/2013/162750
Mantovani, J. R., Carrera, M., Lennon, J., Moreira, A., Marques, J., Bortolotti, A., & Silva, D. A. (2017). Fertility properties and leafy vegetable production in soils fertilized with cattle manure. Revista Caatinga, 30(4), 825–836. http://dx.doi.org/10.1590/1983-21252017v30n402rc
Murakami, K., & Matsuda, R. (2016). Optical and physiological properties of a leaf. In K. Murakami & R. Matsuda (Eds.), LED lighting for urban agriculture (pp. 213 244). Springer.
Mathur, P., Ding, Z., Saldeen, T., & Mehta, J.L. (2015). Tocopherols in the prevention and treatment of atherosclerosis and related cardiovascular disease. Clinical Cardiology, 38(90), 570–576. http://doi.org/10.1002/clc-22422
Mursiani, S., Theresia, M., Budiastuti, S., & Sih, W. (2022). Identification of soybean husk and cow manure metabolites after vermicomposting. International Journal on Advanced Science Engineering Information Technology, 12(4), 1620-1626. http://doi.org/10.18517/ijaseit.12.4.13581
Nguyen, T. (2023). A Review on bioactive compounds and pharmacological properties of Cleome. Journal of Tropical Life Science, 13(3), 615–624. Nurfalah, R., Ridwan, T., Aziz, S.A., Rafi, M., Takemori, H., & Batubara, I. (2024). Growth performance and secondary metabolite production of Adenostemma madurense using different fertilizers. Journal of the Saudi Society of Agricultural Sciences, 23(2), 177–183. https://doi.org/10.1016/j.jssas.2023.10.008
Poli, A., Marangoni, F., Corsini, A., Manzato, E., Marrocco, W., Martini, D., Medea, G., & Visioli, F. (2021). Phytosterols, cholesterol control, and cardiovascular disease. Nutrients Review, 13(8), 1-13. https://doi.org/10.3390/nu13082810
Raisawati, T., Melati, M., Aziz, S.A., & Rafi, M. (2020). Total flavonoid content and antioxidant activity in leaves often cultivated Sonchus arvensis L. accessions. Plant Archives, 20(1), 1785–1792.
Saifulloh, A. A., & Suntari, R. (2022). Growth enhancement, uptake of N, P, and K nutrients, and production of maize in an entisol of Kalidawir, Tulungagung, due to the application of cow manure fertilizer and NPK fertilizer. Jurnal Tanah Dan Sumberdaya Lahan, 9(1), 193–200. https://doi.org/10.21776/ub.jtsl.2022.009.1.21
Sulaeman, Suparto, & Eviati. (2005). Petunjuk teknis analisis kimia tanah, tanaman, air, dan pupuk. Balai Penelitian Tanah. Saputri, L., Hastuti, E. D., & Budihastuti, R. (2018). Respon pemberian pupuk urea dan pupuk kandang sapi terhadap pertumbuhan dan kandungan minyak atsiri tanaman jahe merah [Zingiber officinale (L.) Rosc var. rubrum]. Jurnal Biologi, 7(1), 1–7.
Shàngguān, K.-Y., Huáng, X.-X., Sòng, Y., Zhāng, X.-T., Pān, F., Liú Y.-C., Jiǎng W.-L., & Qián H.-Q. (2020). Effect of different harvesting times on total flavonoid content and antioxidant activity in Rosae chinensis flos. Science and Technology of Food Industry, 41(23), 236–246. http://doi.org/10.13386/j.issn1002-0306.2020020020
Shao, Z., Zhang, X., Nasar, J., & Gitari, H. (2024). Synergetic effect of potassium, biochar, and cattle manure on the growth and yield of maize, and soil physio-chemical characteristics. Plants, 13(23), 1–17. https://doi.org/10.3390/plants13233345
Schütz, V., Frindte, K., Cui, J., Zhang, P., & Hacquard, S. (2021). Differential impact of plant secondary metabolites on the soil microbiota. Frontier in Microbiology, 12, 1–17. https://doi.org/10.3389/fmicb.2021.666010
Shilla, O., Dinssa, F. F., Omundi, E. O., Traud, W., & Onyango, M. O. A. (2019). Cleome gynandra L. origin, taxonomy, and morphology: A review. African Journal of Agricultural Research, 14(32), 1568–1583. https://doi.org/10.5897/ajar2019.14064
Sofian, A., Nuraida, & Tumanggor, D. S. (2024). Pemanfaatan pupuk sapi kandang dan pupuk cair organik kulit pisang untuk meningkatkan pertumbuhan dan produksi jagung manis (Zea mays L. saccharata Sturt). G-Tech : Jurnal Teknologi Terapan, 8(1), 453–463. https://doi.org/10.33379/gtech.v8i1.3794
Valitova, J., Renkova, A., & Beckett, R. (2024). Stigmasterol : An enigmatic plant stress sterol with versatile functions. International Journal of Molecular Sciences Review, 25(15), 1-19. https://doi.org/10.3390/ijms25158122
Virsangbhai, C. K., Goyal, A., Tanwar, B., & Sihag, M. K. (2020). Potential health benefits of conjugated linoleic acid : An important functional dairy ingredient. European Journal of Nutrition & Food Safety, 11(4), 200–213. https://doi.org/10.9734/EJNFS/2019/v11i430162
Vongsak, B., Thongpraditchote, S., Mangmool, S., & Wongkrajang, Y. (2013). Maximizing total phenolics, total flavonoid contents, and antioxidant activity of Moringa oleifera Leaf. Industrial Crops & Products, 44, 566–571. https://doi.org/10.1016/j.indcrop.2012.09.021
Whalen, J. K., Chang, C., & Clayton, G. W. (2002). Cattle manure and lime amendments to improve crop production of acidic soils in northern Alberta. Canadian Journal of Soil Science, 82(2), 227-238. http://doi.org/10.4141/S01-030
Downloads
Published
How to Cite
Issue
Section
License
All publications by Journal of Tropical Crop Science is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.



