Cytological Deterioration of Bitter Gourd (Momordica charantia L.) Pollen During Storage and Its Impact on Effectiveness for Seed Production
DOI:
https://doi.org/10.29244/jtcs.12.02.303-313Keywords:
intine, mitochondria, pollen viability, seed quality, seed setAbstract
Pollen storage plays an important role in the hybrid seed production of bitter gourd, but a primary challenge is the rapid decline in pollen viability. This research aimed to investigate the mechanisms of bitter gourd pollen deterioration during storage and to assess the effectiveness of stored pollen for hybrid seed production. The study was conducted at the Leuwikopo Research Station, IPB University, from September 2018 to June 2021. Bitter gourd pollen was collected at anthesis and stored in a deep freezer at -21±3ºC. Pollen viability was observed at 0, 2, 4, 6, 8, 10, and 12 weeks after storage (WAS), while pollen ultrastructure was examined at 0, 4, 8, and 12 WAS using a transmission electron microscope. The stored pollen was then used for pollination, observations were made on fruit set, seed set, and seed quality. The viability of bitter gourd pollen declined rapidly following dehydration before storage. The declining pollen viability during storage was due to the degeneration of the intine, followed by mitochondrial deterioration. The cristae became disorganised, leading to the dissolution of the cristae and outer membrane of mitochondria. Storing the pollen in a deep freezer (-21±3ºC) after dehydration did not prevent a further decline in pollen viability. Bitter gourd pollen stored for more than 2 weeks at -21±3ºC is ineffective for seed production. Cucurbit pollen remains viable for only two weeks; thus, fresh pollen is more practical for hybrid seed production. Development of more effective pollen storage methods is necessary to prolong pollen viability and enhance breeding efficiency.
References
Agustin, H., Palupi, E.R., and Suhartanto, M.R. (2014). Pollen management for seed production of Sunrise Meta and Orange Meta hybrid melon. Jurnal Hortikultura 24, 32–41.
Ahmad, N., Hasan, N., Ahmad, Z., Zishan, M., and Zohrameena, S. (2016). Momordica charantia: for traditional uses and pharmacological actions. Journal of Drug Delivery and Therapeutics 6, 40–44. DOI: https://doi.org/10.22270/jddt.v6i2.1202.
Akutsu M. (2016). Effective long-term storage methods for pollen in bitter gourd (Momordica charantia L.). In "Proceeding of Cucurbitaceae, the XIth EUCARPIA Meeting on Cucurbit Genetics and Breeding" (E.U. Kozik, H.S. Paris, eds.). July 24–28, 2016, Warsaw, Poland. pp 197–198. DOI: https://doi.org/10.5281/zenodo.1255670.
Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2002). "Molecular Biology of the Cell". 4th edition. Garland Science. New York.
Anusree, T., Abhina, C., Lishiba, P.P., Rasna, T.V., Varma, S., and Sinu, P.A. (2015). Flower sex expression in cucurbit crops of Kerala: implications for pollination and fruit set. Current Science 109, 2299-2302. DOI: https://doi.org/10.18520/cs/v109/i12/2299-2302.
Ashok, A.D., Kayalvizhi, K., and Ravivarman, J. (2020). Influence of ethrel on sex expression in cucurbitaceous vegetables. International Journal of Chemical Studies 8, 851–856. DOI: https://doi.org/10.22271/chemi.2020.v8.i4e.9713.
Behera, T.K., John, J.K., Simon, J.E., Staub, P.W. (2010). Bitter gourd: botany, horticulture, and breeding.Horticultural Reviews 37, 102-141.DOI: https://doi.org/10.1002/9780470543672.ch2.
Chaanine, A.H. (2019). Morphological stages of mitochondrial vacuolar degeneration in phenylephrine-stressed cardiac myocytes and in animal models and human heart failure. Medicina 55, 239-257. DOI: https://doi.org/10.3390/medicina55060239.
Chen, J.C., Bik-San Lau, C., Chan, J.Y.W., Fung, K.P., Leung, P.C., Liu, J.Q., Zhou, L., Xie, M.J., and Qiu, M.H. (2015). The Antigluconeogenic activity of cucurbitacins from Momordica charantia. Planta Medica 81, 327-332. DOI: https://doi.org/10.1055/s-0035-1545695.
Craeye, S., Bleyaert, P., De Storme, N., and Van Labeke, M.C. 2020. Evaluation of pollen storage strategies in Cucurbita pepo L. Acta Horticulturae 1271, 379-385. DOI: https://doi.org/10.17660/ActaHortic.2020.1271.52.
Deyto, R. C., and Cervancia, C. R. (2009). Floral biology and pollination of Ampalaya (Momordica charantia L.). Philippine Agricultural Scientist 92, 8-18.
Fang, E.F., Zhang, C.Z.Y., Wong, J.H., Shen, J.Y., Li, C.H., and Ng, T.B. (2012). The MAP30 protein from bitter gourd (Momordica charantia) seeds promotes apoptosis in liver cancer cells in vitro and in vivo. Cancer Letters 324, 66–74. DOI: https://doi.org/10.1016/j.canlet.2012.05.005.
Fariroh, I., Palupi, E.R., and Wahyudin, D.S. (2012). Germination medium and storage condition of Cucumis sativus pollen. In "Proceeding of National Seminar of Indonesian Association for Horticulture 2011". Lembang, West Java, Indonesia: PERHORTI. pp. 431–438.
Fayos, O., Echávarri, B., Vallés, M. P., Mallor, C., Garcés-Claver, A., and Castillo, A. M. (2022). A simple and efficient method for onion pollen preservation: germination, dehydration, storage conditions, and seed production. Scientia Horticulturae 305, 111358. DOI: https://doi.org/10.1016/j.scienta.2022.111358.
Franchi, G.G., Piotto, B., Nepi, M., Baskin, C.C., Baskin, J.M., and Pacini, E. (2011). Pollen and seed desiccation tolerance in relation to degree of developmental arrest, dispersal, and survival. Journal of Experimental Botany 62, 5267–5281. DOI: https://doi.org/10.1093/jxb/err154.
Garcia, C.C., Guarnieri, M., and Pacini, E. (2015). Carbohydrate metabolism before and after dehiscence in the recalcitrant pollen of pumpkin (Cucurbita pepo L.). Plant Biology 17, 734–739. DOI: https://doi.org/10.1111/plb.12279.
Halbritter, H. (2015). Momordica charantia In "A Palynological Database". https://www.paldat.org/pub/Momordica_charantia/300075;jsessionid=A7E1475BFE49789279236CAA830091B0. [June 3, 2022].
Halbritter, H., Ulrich, S., Grímsson, F., Weber, M., Zetter, R., Hesse, M., Buchner, R., Svojtka, M., and Frosch-Radivo, A. (2018). "Palynology: History and Systematic Aspects. Springer, Switzerland. DOI: https://doi.org/10.1007/978-3-319-71365-6_1.
Harliani, E.N., Palupi, E.R., and Wahyudin, D.S. (2014). The potential of pollen storage for hybrid seed production of cucumber (Cucumis sativus L.) variety KE014. Jurnal Hortikultura Indonesia 5, 104–117. DOI: https://doi.org/10.29244/jhi.5.2.104-117.
Ikon, N., and Ryan, R.O. (2017). Cardiolipin and mitochondrial cristae organisation. Biochimica et Biophysica Acta (BBA) - Biomembranes 1859, 1156–1163. DOI: https://doi.org/10.1016/ j.bbamem.2017.03.013.
[ISTA] International Seed Testing Association. (2018). "International Rules for Seed Testing." Zurich, Switzerland.
Jia, W.Q., Wang, Y.L., Guo, Y.Z., Wang, Z., Qi, Q., Yan S.N., Liu H.C., and He S.L. (2021). Characterisation of pollen germination and storage of Paeonia ludlowii. Scientiae Silvae Sinicae 57, 82–92. https://doi.org/10. 11707/j. 1001- 7488. 20210 209.
Liu S., Li Z., Wu S., and Wan, X. (2021). The essential roles of sugar metabolism for pollen development and male fertility in plants. The Crop Journal 9, 1223–1236. DOI: https://doi.org/10.1016/j.cj.2021.08.003
Matsumura, H., Urasaki, N., Pandey, S., and Gautam, K.K. (2020). Sex determination in bitter gourd In "The Bitter Gourd Genome, Compendium of Plant Genomes" (C. Kole, H. Matsumura, T. Behera, eds.). Springer. DOI: https://doi.org/10.1007/978-3-030-15062-4_6.
Mendez, N.P., and Acma, F.M. (2018). In vitro studies on pollen viability, pollen germination, and pollen tube growth of Hornstedtia conoidea Ridl., a Philippine endemic ginger species. Journal of Tropical Life Science 8, 303-310. DOI: https://doi.org/10.11594/jtls.08.03.13.
Moore, J.P., Vicre´-Gibouinb, M., Farrantc, J.M., and Driouichb, A. (2008). Adaptations of higher plant cell walls to water loss: drought versus desiccation. Physiologia Plantarum 134, 237–245. DOI: https://doi.org/10.1111/j.1399-3054.2008.01134.x.
Nagar, A., Gowthami, R., Sureja, A.K., Munshi, A.D., Verma, M., Singh, A.K., Mallick, N., Singh, J., Chander, S., Shankar, M., Pathania, P., and Rajkumar S. (2023). Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency. Frontier Plant Science 14, 1268726. DOI: https://doi.org/10.3389/fpls.2023.1268726.
Nepi, M., Cresti, L., Guarnieri, M., and Pacini, E. (2010). Effect of relative humidity on water content, viability, and carbohydrate profile of Petunia hybrida and Cucurbita pepo pollen. Plant Systematics and Evolution 284, 57–64. DOI: https://doi.org/10.1007/s00606-009-0237-x.
Oliver, M.J., Farrant, J.M., Hilhorst, H.W.M., Mundree, S., Williams, B., and Bewley, J.D. (2020). Desiccation tolerance: avoiding cellular damage during drying and rehydration. Annual Review Plant Biology 29, 435-460. DOI: https://doi.org/10.1146/annurev-arplant-071219-105542.
Pacini, E., and Dolferus, R. (2019). Pollen developmental arrest: Maintaining pollen fertility in a world with a changing climate. Frontier Plant Science 10, 679. DOI: https://doi.org/10.3389/fpls.2019.00679.
Palupi, E.R., Sidabutar, R.M., and Wanafiah, K. (2017). Pollen drying and storage for hybrid seed production of cucumber (Cucumis sativus L.). Acta Horticulturae 1151, 163–168. DOI: https://doi.org/10.17660/ActaHortic.2017.1151.26.
Pradeepkumar, T., and Lekshmanan, D.K. (2024). Mechanism of hybrid seed production in cucurbitaceous vegetables. Euphytica 220, 17. https://doi.org/10.1007/s10681-023-03278-y
Srivastava, A., and Sharma, S. (2016). Pollen morphology of some cultivated cucurbits of Ganga Plain. Geophytology 46, 15–23.
Suhri, A.G.M.I, RC Hidayat Soesilohadi, R.C.H, Putra, R.E., Raffiudin, R., Purnobasuki, H., Agus, A., and Kahono, S. (2022). The effectiveness of stingless bees on pollination of bitter melon plants Momordica charantia L. (Cucurbitaceae). Journal of Tropical Biodiversity and Biotechnology 7, jtbb69124. DOI: https://doi.org/10.22146/jtbb.69124.
Sumiati, E., Susilo, F. X., and Widodo, M. S. (2019). Diversity of bee pollinators and their role in bitter gourd (Momordica charantia L.) pollination in West Java, Indonesia. AGRIVITA Journal of Agricultural Science 41, 592-602. doi: 10.17503/agrivita.v41i3.214
Tran, X.T., Parks, S.E., Nguyen, M.H., and Roach, P.D. (2021). Reduced pollination efficiency compromises some physicochemical qualities in Gac (Momordica cochinchinensis Spreng.) fruit. Agronomy 11, 1–12. DOI: https://doi.org/10.3390/agronomy11010190.
Wang, S., Zhang, Y., Song, Q., Fang, Z., Chen, Z., Zhang, Y., Zhang, L., Zhang, L., Niu, N., Ma, S., Wang, J., Yao, Y., Hu, Z., and Zhang, G. (2018). Mitochondrial dysfunction causes oxidative stress and tapetal apoptosis in chemical hybridization reagent-induced male sterility in wheat. Frontier Plant Science 8, 2217. DOI: https://doi.org/10.3389/fpls.2017.02217.
Williams, J.H., and Brown, C.D. (2018). Pollen has higher water content when dispersed in a tricellular state than in a bicellular state. Acta Botanica Brasilica 32, 454-461. DOI: https://doi.org/10.1590/0102-33062018abb0129.
Yin, G., Whelan, J., Wu, S., Zhou, J., Chen, B., Chen, X., Zhang, J., He, J., Xin, X., and Lu, X. (2016). Comprehensive mitochondrial metabolic shift during the critical node of seed ageing in rice. PLOS ONE 11, e0148013. DOI: https://doi.org/10.1371/journal.pone.0148013.
Yogapriya, A., Usharani, B., Suresh, K., Vellaikumar, S., and Chinniah, C. (2019). Foraging behavior of major pollinators in bitter gourd. International Journal of Current Microbiology and Applied Science 8, 947-954. DOI: https://doi.org/10.20546/ijcmas.2019.806.114.
Zurbano, L., Bellere, A., and de Asis, G. (2021). Growth, yield, and quality of bitter gourd (Momordica charantia L.) under organic fertilisation schemes. Journal of Applied Horticulture 23, 193-199. DOI: https://doi.org/10.37855/jah.2021.v23i02.36.
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